US app 20150118735 to Heliae: METHODS OF CULTURING MICROORGANISMS IN NON-AXENIC MIXOTROPHIC CONDITIONS

First claim of --METHODS OF CULTURING MICROORGANISMS IN NON-AXENIC MIXOTROPHIC CONDITIONS --

A method of culturing Chlorella in non-axenic mixotrophic culture conditions, comprising inoculating an aqueous culture medium with a culture comprising Chlorella in an open culturing vessel in the presence of: a. light comprising photosynthetically active radiation (PAR); b. an organic carbon source comprising at least one selected from the group consisting of acetic acid, acetate, and acetic anhydride, at a concentration in the range of 15-50% (v/v); c. a pH in the range of 6-9; d. an initial concentration of sodium acetate, sodium hydroxide, or potassium hydroxide in the range of 0.05-10 g/L; and e. a dissolved oxygen (DO) concentration in the range of 1-6 mg O.sub.2/Liter of culture.