Joule Unlimited's US application 20150159178: Ethanol Production in Microorganisms

The first claim of US 20150159178:

A method for production of ethanol, comprising: culturing an engineered photosynthetic microbe in a culture medium in the presence of light and inorganic carbon, wherein said engineered photosynthetic microbe comprising a recombinant pyruvate decarboxylase nucleic acid sequence encoding a pyruvate decarboxylase and a recombinant alcohol dehydrogenase nucleic acid sequence encoding an alcohol dehydrogenase, wherein the expression level the alcohol dehydrogenase and the expression level of the pyruvate decarboxylase are regulated by separate promoters, wherein the expression level of alcohol dehydrogenase in the engineered photosynthetic microbe is greater than the expression level of alcohol dehydrogenase in a control engineered photosynthetic microbe comprising a recombinant alcohol dehydrogenase nucleic acid sequence and a recombinant pyruvate decarboxylase nucleic acid sequence regulated together by a single promoter, and wherein the engineered photosynthetic microbe produces ethanol in an amount greater than a non-engineered photosynthetic microbe, when cultured under identical conditions.